HPLC (high-pressure liquid chromatography) is a chromatographic technique that can isolate a mixture of compounds and is used to classify, measure, and purify the individual components of the mixture in biochemistry and analytical chemistry. HPLC usually uses various types of stationary phases, a pump to drive the mobile phase(s) and analyte through the column, and a detector to provide the analyte with a characteristic retention time. If fitted, the detector can also provide additional information (for example, UV/Vis spectroscopic data for the analyte). The frequency of the analyte's interactions with the stationary phase, the ratio/composition of the solvent(s) used, and the flow rate of the mobile phase all influence the analyte's retention time. Because of the broad range of separation principles, HPLC (high-performance liquid chromatography) can be used for all biochemical studies (adsorption-, partition-, ion-exchange- and steric exclusion-chromatography). The reversed phase technique, a form of partition chromatography, is particularly important. The sensitivity of detectors varies depending on the structure of the material, but it is usually lower than GLC-detectors (apart from fluorescence detection). New technologies are currently being developed. The HPLC is used to determine the quantitative levels of drugs and their metabolites in the blood. This is particularly critical when designing new medications and tracking treatment. The identification of these biogenic substances is assisted by the calculation of their concentrations. The use of HPLC in sample clean-up for other analytical methods is an important area of application. Because of its high data reproducibility and ease of automation, high performance liquid chromatography (HPLC) has been commonly used in clinical laboratory studies. The HPLC method was used to isolate IgG and albumin in commercially available IgG preparations, as well as to assess blood theophylline and serum uric acid levels. To accurately calculate the whole blood uric acid content, samples were pretreated for deproteinization by acid before being tested for serum uric acid. To increase affinity to the reversed column's ODS phase, the eluent was changed to pH 2.2. (TSK gel ODS-80 TM). Between HPLC and the uricase-catalase process, there was a strong correlation of uric acid values. Those who are interested to submit their manuscript in our journal for publication, the can submit it either online through given link: https://www.longdom.org/submissions/clinical-chemistry-laboratory-medicine.html or send it to us as an email attachment to below given mail id.

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Journal of Clinical chemistry and Laboratory Medicine

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